BACTERIAL CLEANSING
Standout bacteria reduction.
In the study's single-rinse comparison, TRI-OLOGY® showed the greatest overall reduction in measured bacterial DNA among the rinses evaluated.
MEASURABLE PERFORMANCE AFTER ONE RINSE
TRI-OLOGY® SCIENCE & RESEARCH
Powerful Cleansing. Biology-Conscious Care.
TRI-OLOGY® developed from early formulation research into professional dental evaluations, university-based antimicrobial screening, analytical chemistry, and published comparative testing. The materials below explain what each investigation actually measured, what it found, and where further clinical research is still needed.
2015 PUBLISHED COMPARATIVE RESEARCH
Powerful Cleansing. Remarkable Oral-Cell Results.
Published comparative research examined two essential sides of oral care: reducing measured oral bacteria and studying how living gingival cells respond to cleansing formulations.
ORAL-CELL VIABILITY
Living cells made the difference.
In the reported laboratory comparison, TRI-OLOGY® was the only tested rinse to retain measurable gingival fibroblast viability under the study conditions.
A DIFFERENT APPROACH TO ORAL CLEANSING
EXPLORE THE PUBLISHED FINDINGS
One study. Two compelling results.
Bowen J, Cole C, McGlennen R. Comparison of Antimicrobial and Wound Healing Agents on Oral Fibroblast Viability and In-vivo Bacterial Load. Dentistry, 2015.
The published study contains methods, sample details and complete results for readers who want to explore the science further.
ORIGINAL TEST REPORTS
Four Research Milestones. Remarkable Results.
From university testing to independent laboratory reports and dental case documentation, TRI-OLOGY® has a research history worth exploring. Discover four standout findings from the studies and reports behind our formulas.
01 / UNIVERSITY DENTAL LAB2012
Powerful Antimicrobial Activity. Verified in the Lab.
GROWTH INHIBITEDAgainst tested bacteria linked to oral disease
University dental-lab testing found TRI-OLOGY® Professional Strength Oral Serum inhibited bacteria associated with gum disease and tooth decay.
LAB-VERIFIED ANTIMICROBIAL ACTIVITY
Source: Richard L. Gregory, PhD, In Vitro Analysis of Antimicrobial Activity of Wheat Grass-Containing Solutions (in-vitro final report, received 2012).02 / ANALYTICAL CHEMISTRY2015
Inside Our Plant-Powered Formula
20constituents identified by GC-MS and LC-MS
MRIGlobal identified 20 constituents in TRI-OLOGY® Debriding Oral Rinse using GC-MS and LC-MS techniques, including vitamin E, luteolin, chlorogenic acid and menthol.
Independent formula-constituent analysis · MRIGlobal
Source: MRIGlobal, Determining Antioxidant Content of TRIOLOGY Debriding Oral Rinse, report dated January 23, 2015.03 / VIRUS SUSPENSION TEST2020
Independently Measured Viral Reduction
≥3.10log₁₀ reduction in a 2-minute test
Microbac Laboratories documented at least a 3.10-log₁₀ reduction in infectious SARS-CoV-2 during a controlled two-minute suspension test of a TRI-OLOGY® 4% carbamide-peroxide rinse formulation.
In-vitro result; not evidence of COVID-19 prevention or treatment in people.
Source: Microbac Laboratories, audited report, project 1031-101, signed November 3, 2020.04 / REAL-WORLD DENTAL CARE2020
Real Patients. Dentist-Documented Results.
DOCUMENTEDClinical case experiences in dental practice
Dentist Dr. James D. Elias documented TRI-OLOGY® use during dental care, capturing treatment and follow-up photographs that tell the story of patients' experiences. His case work was featured in RDH.
REAL PATIENT CASES · DENTIST-DOCUMENTED PHOTOGRAPHY
Source: James D. Elias, DDS, Case studies with Tri-ology rinse, RDH, June 2020 (sponsored article), plus historical case photographs.Our formulas have stood the test of time.
The TRI-OLOGY® Rinse and Serum formulas featured in these research reports are the same formulations used today—reflecting our commitment to consistent, research-informed oral care.
A DIFFERENT FORMULATION PHILOSOPHY
Biology First. Chemistry With Purpose.
Your mouth is a living environment—not an inert surface. TRI-OLOGY® combines purposeful oral-care chemistry with xylitol and carefully selected plant-derived ingredients in an alcohol-free formula designed with the biology of the mouth in mind.
Carbamide peroxide
Purposeful oral-care chemistry for micro-foaming cleansing and cosmetic whitening as part of the complete Restoring Rinse® formula.
Wood-derived xylitol
A well-established oral-care ingredient derived from hardwood and incorporated intentionally into the daily formula.
Organic aloe + wheatgrass
Plant-derived ingredients selected as part of TRI-OLOGY®’s broader whole-mouth formulation philosophy.
Organic peppermint + sweet almond
Plant-derived oils support freshness, sensory experience and mouthfeel. Contains sweet almond oil.
Always review the current product label for the complete ingredient list, directions and warnings.
A SCIENCE STORY BUILT OVER TIME
Professional use. Laboratory testing. Published research.
From an oral-health research idea in 2001 to decades of formulation work, dental evaluations, published research and patents, TRI-OLOGY® has continued to evolve. These are selected milestones from company records and published sources.
A research question becomes a mission
Janice King and Dale Winetroub begin investigating the relationship between oral health, cleansing methods and the body's responses to oral irritation.
Sixty formulations screened
Historical training materials describe five screening rounds of 12 candidate formulations while developing the combination concept.
Professional dental evaluations begin
Company records report TRI-OLOGY® use and practitioner evaluations in dental settings beginning in March 2011.
University antimicrobial report
University dental-lab testing documented bacterial-growth inhibition with TRI-OLOGY® Professional Strength Oral Serum.
Independent chemistry analysis
MRIGlobal documents chemical constituents detected in a sample of historical Debriding Oral Rinse using GC-MS and LC-MS techniques.
Comparative findings published
Bowen, Cole and McGlennen report an oral bacterial-DNA pilot and gingival fibroblast laboratory experiments in Dentistry.
Three U.S. patents issued
Patents 8,961,939; 9,339,453; and 9,668,962 mark stages of the company's intellectual-property development—not clinical approval.
Microbac virus suspension testing
A historical TRI-OLOGY® rinse formulation is assessed in a controlled two-minute SARS-CoV-2 laboratory assay.
Dental case experiences published
Dr. James D. Elias publishes a sponsored case-study article featuring TRI-OLOGY® in RDH.
Research informs ongoing product development
Restoring Rinse® builds on a longer formulation history while independent evidence, limitations and formulation differences remain important.
THE TRI-OLOGY® SCIENCE
Microbiology + Chemistry + Tissues.
Microbiology
The mouth contains complex microbial communities. TRI-OLOGY® approaches cleansing within that larger oral environment.
Chemistry
Purposeful ingredients such as 4% carbamide peroxide deliver micro-foaming cleansing and cosmetic whitening as part of the complete formula.
Tissues
Daily oral-care products come in direct contact with living oral tissues. TRI-OLOGY® believes tissue response deserves consideration alongside cleansing performance.
EXPLORE THE EVIDENCE
Original research. Independent perspectives. Better questions.
Explore the published comparative study, university and independent laboratory testing, dentist-documented case experiences, and additional research supporting the TRI-OLOGY® approach.
TRI-OLOGY® original sources
Emerging oral-health research
View all articlesResearch resources include published studies, original laboratory reports, and practitioner case observations. Each source provides methods, context and additional findings for readers who want to explore further.
OUR FLUORIDE-FREE PLEDGE
Fluoride Free.
By Choice. By Design.
Fluoride exposure comes from more than one place: drinking water, food and beverages, toothpaste, and some mouthwashes. For most people, knowing their exact daily total isn't simple.
That's why TRI-OLOGY® makes a deliberate choice: we do not add fluoride to our Rinse and Serum formulations.
Understand everyday fluoride sourcesFluoride remains an established option for cavity prevention. Our formulation pledge is a consumer-choice commitment, not a recommendation to stop professionally advised fluoride care. Ask your dental professional which approach fits your needs.
NEW INDEPENDENT U.S. RESEARCH
Higher Prenatal Fluoride Exposure Linked to Lower Fluid Cognition.
A 2026 study involving 2,514 children found that higher estimated prenatal fluoride exposure from public drinking water was associated with lower fluid-cognition scores—skills related to attention, memory and flexible thinking.
The findings add important U.S. evidence to ongoing research on fluoride exposure and the developing brain.
2,514Children included
23U.S. states represented
Key finding: Higher estimated prenatal exposure was associated with lower fluid-cognition scores.
An observational study of estimated prenatal drinking-water exposure. Explore the original research for methodology, statistical analyses and interpretation.
Research findings above reflect the specific study designs, methods and conditions reported in the cited research. See the original publication for complete methodology and findings.
FROM SCIENCE TO YOUR DAILY ROUTINE
A more intelligent way to care for your mouth.
Restoring Rinse® brings effective cleansing, fresh breath, brighter-looking teeth, plant-derived ingredients and alcohol-free comfort together in one daily rinse—built around a more complete view of the oral environment.